胚胎移植培养基 EmbryoGlue 对卵裂期冻融胚胎移植临床结局的影响

巧燕 王(温州医科大学附属东阳医院生殖中心，中国)
玲玲 金(温州医科大学附属东阳医院生殖中心，中国)
梦婷 周(温州医科大学附属东阳医院生殖中心，中国)
华卿 厉(温州医科大学附属东阳医院生殖中心，中国)

摘要

目的：探讨胚胎移植培养基 EmbryoGlue 对卵裂期冻融胚胎移植临床结局的影响。方法：选取2020年8月1日至2022年12月31日期间在浙江省东阳市人民医院生殖中心接受第3天卵裂期冻融胚胎移植的患者作为研究对象。根据移植培养基的类型，将患者分为G-2组和EmbryoGlue组。分析患者在激素替代治疗前（基线）及治疗后的实验室检测报告和超声结果，并通过单变量分析和二元回归分析比较两组的临床妊娠结局。结果：共纳入333个周期，其中G-2组162个周期，EmbryoGlue组171个周期。G-2组的临床妊娠率为56.79%，EmbryoGlue组为56.73%（P = 0.990），无显著差异。G-2组的活产率为48.77%，EmbryoGlue组为47.37%（P = 0.799），无统计学意义。在三个亚组（高龄（≥ 35岁）与非高龄（< 35岁）、IVF与ICSI、H RT与GnRH-HRT）中进行的Logistic回归分析发现，EmbryoGlue移植与G-2移植与患者的临床妊娠率和活产率之间无统计学关联。结论：在冻融胚胎移植中，EmbryoGlue移植培养基与G2培养基具有相似的临床结局。

关键词

EmbryoGlue；胚胎移植；临床妊娠率；活产率；卵裂期胚胎

参考

Gardner DK, Lane M. Blastocyst transfer. Clin Obstet Gynecol 2003;46:231–8. https://doi.org/10.1097/00003081-200306000-00005.

Bontekoe S, Blake D, Heineman MJ, Williams EC, Johnson N. Adherence compounds in embryo transfer media for assisted reproductive technologies. Cochrane Database Syst Rev 2010:CD007421. https://doi.org/10.1002/14651858.CD007421.pub2.

Carson DD, Dutt A, Tang JP. Glycoconjugate synthesis during early pregnancy: Hyaluronate synthesis and function. Dev Biol 1987;120:228–35. https://doi.org/10.1016/0012-1606(87)90120-5.

Gardner DK, Rodriegez-Martinez H, Lane M. Fetal development after transfer is increased by replacing protein with the glycosaminoglycan hyaluronan for mouse embryo culture and transfer. Hum Reprod 1999;14:2575–80. https://doi.org/10.1093/humrep/14.10.2575.

Chun S, Seo JE, Rim YJ, Joo JH, Lee YC, Koo YH. Efficacy of hyaluronan-rich transfer medium on implantation and pregnancy rates in fresh and frozen-thawed blastocyst transfers in korean women with previous implantation failure. Obstet Gynecol Sci 2016;59:201–7. https://doi.org/10.5468/ogs.2016.59.3.201.

Fancsovits P, Lehner A, Murber A, Kaszas Z, Rigo J, Urbancsek J. Effect of hyaluronan-enriched embryo transfer medium on IVF outcome: A prospective randomized clinical trial. Arch Gynecol Obstet 2015;291:1173–9. https://doi.org/10.1007/s00404-014-3541-9.

Simon A, Safran A, Revel A, Aizenman E, Reubinoff B, Porat-Katz A, et al. Hyaluronic acid can successfully replace albumin as the sole macromolecule in a human embryo transfer medium. Fertil Steril 2003;79:1434–8. https://doi.org/10.1016/s0015-0282(03)00349-2.

Singh N, Gupta M, Kriplani A, Vanamail P. Role of embryo glue as a transfer medium in the outcome of fresh non-donor in-vitro fertilization cycles. J Hum Reprod Sci 2015;8:214–7. https://doi.org/10.4103/0974-1208.170398.

Valojerdi MR, Karimian L, Yazdi PE, Gilani MAS, Madani T, Baghestani AR. Efficacy of a human embryo transfer medium: A prospective, randomized clinical trial study. J Assist Reprod Genet 2006;23:207–12. https://doi.org/10.1007/s10815-006-9031-7.

Yung SSF, Lai SF, Lam MT, Lui EMW, Ko JKY, Li HWR, et al. Hyaluronic acid-enriched transfer medium for frozen embryo transfer: A randomized, double-blind, controlled trial. Fertil Steril 2021;116:1001–9. https://doi.org/10.1016/j.fertnstert.2021.02.015.

Bhoi NR, Murdia N, Murdia K, Chandra V, Suwalka I, Mistari W, et al. Effect of hyaluronic acid-containing transfer media (EmbryoGlue®) on the live birth rate in frozen thawed embryo transfer cycles. Cureus 2024;16:e52713. https://doi.org/10.7759/cureus.52713.

Dong L, Lian F, Wu H, Xiang S, Li Y, Wei C, et al. Reproductive outcomes of dual trigger with combination GnRH agonist and hCG versus trigger with hCG alone in women undergoing IVF/ICSI cycles: A retrospective cohort study with propensity score matching. BMC Pregnancy Childbirth 2022;22:583. https://doi.org/10.1186/s12884-022-04899-2.

DOI: http://dx.doi.org/10.12345/yzlcyxzz.v3i10.33028