硼[10B] 法仑对 U87 细胞毒性及滞留时间的影响

水英 罗(厦门弘爱医院，中国;)
俊强 洪(厦门弘爱医院，中国;)
晓萍 孙(中硼（厦门）生物医药有限公司，中国;)
平 周(中硼（厦门）生物医药有限公司，中国;)

摘要

目的：明确HMC3、U87 L-苯丙氨酸与硼[10B]法仑的细胞毒性及L-苯丙氨酸对硼[10B]法仑滞留时间的影响。方法：细胞培养后分别进行L-苯丙氨酸与硼[10B]法仑细胞毒性检测。根据毒性阈值，设置一定的浓度进行L-苯丙氨酸刺激、孵育硼[10B]法仑评估HMC3、U87细胞硼浓度。结果：HMC3、U87硼[10B]法仑细胞毒性阈值分别为3200ppm与2600ppm。其L-苯丙氨酸细胞毒性阈值均为20mM。HMC3、U87细胞先孵育2500ppm 硼[10B]法仑，后分组进行再孵育5mM L-苯丙氨酸，发现HMC3硼浓度变化不明显，U87硼浓度较高，但没有统计学差异。后进行不同浓度和时间的L-苯丙氨酸孵育，U87孵育L-苯丙氨酸组较无苯丙氨酸组硼浓度显著增加，HMC3未见显著差异，且U87硼浓度高于HMC3。结论：U87经硼[10B]法仑孵育及一定浓度的L-苯丙氨酸刺激后其硼浓度高于HMC3。

关键词

硼[10B]法仑；L-苯丙氨酸；U87；HMC3；细胞毒性；硼浓度

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DOI: http://dx.doi.org/10.12345/yzlcyxzz.v3i10.33034