亚甲基四氢叶酸还原酶基因多态性与糖尿病肾病的相关性研究

洁 程(广东医科大学第二临床医学院，中国;)
伟 赵(东莞市台心医院内科，中国;)
峰 许(东莞市台心医院内科，中国;)
吟 程(广东医科大学第二临床医学院，中国;)

摘要

目的：探讨亚甲基四氢叶酸还原酶（MTHFR）基因多态性与糖尿病肾病（DN）的关系。方法：采用标准的酚-氯仿-蛋白酶K法提取外周血DNA；分别用PCR—RFLP法检测50例正常对照组、51例糖尿病肾病（DN）组和47例糖尿病非肾病（非DN）组MTHFR基因C677T多态性；遗传平衡吻合度检验用Hardy-Weinberg平衡法。采用卡方检验分析基因多态性与DN的相关性。并比较各组间MTHFR基因C677T基因型频率和等位基因频率。结果：①各病例组MTHFR基因C677T的CT基因型及T等位基因频率显著高于对照组，差异有显著性（P<0.05）；②病例组之间MTHFR基因型及T等位基因频率差异无显著性（P<0.05）。结论：MTHFR基因C677T多态性与糖尿病肾病并发症密切相关。

关键词

糖尿病；亚甲基四氢叶酸还原酶；基因频率；基因多态性

参考

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DOI: http://dx.doi.org/10.12345/yzlcyxzz.v8i6.28995