法医物证中尿液与精液 mRNA 差异表达分析

高原 宋(深圳市公安局刑事侦查支队刑事技术处，中国)
路 尹(深圳市公安局刑事侦查支队刑事技术处，中国)
舟 杜(深圳市公安局刑事侦查支队刑事技术处，中国)
湘秦 李(深圳市公安局刑事侦查支队刑事技术处，中国)

摘要

目的：探索法医物证中尿液与精液 mRNA 表达差异，筛选特异基因用于组织来源检验鉴定。方法：对尿液和精液进行微量RNA 建库测序，经生物信息学分析差异表达基因，筛选组织特异性高表达基因。结果：在尿液和精液中分别检测到9639和14660个表达基因，进一步分析发现在尿液和精液中分别有816个和5837个特异表达基因。通过GO富集发现，在尿液中特异表达基因富集在免疫反应、细胞排泄等生物学过程，检测到BHMT、PTH1R、HAO2、SULT1A2呈高表达；精液中特异表达基因富集在精子发育、精子运动等生物学过程，PGM3、PRM1、SMCP、KLK2为高表达基因。结论：通过对尿液和精液测序分析发现，BHMT、PTH1R、HAO2、SULT1A2在尿液中特异性高表达，PGM3、PRM1、SMCP、KLK2在精液中特异高表达，可作为二者组织特异性表达鉴定。

关键词

法医物证，尿液，精液，mRNA差异表达

参考

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DOI: http://dx.doi.org/10.12345/yzlcyxzz.v8i12.34986